A DNA Vaccine Against Infectious Hematopoietic Necrosis Virus
نویسندگان
چکیده
منابع مشابه
Plaquing procedure for infectious hematopoietic necrosis virus.
A single overlay plaque assay was designed and evaluated for infectious hematopoietic necrosis virus. Epithelioma papillosum carpio cells were grown in normal atmosphere with tris(hydroxymethyl)aminomethane- or HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid)-buffered media. Plaques were larger and formed more quickly on 1- to 3-day-old cell monolayers than on older monolayers. Cell ...
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The genome of infectious hematopoietic necrosis virus (IHNV), a salmonid novirhabdovirus, has been engineered to modify the gene order and to evaluate the impact on a possible attenuation of the virus in vitro and in vivo By reverse genetics, eight recombinant IHNVs (rIHNVs), termed NxGy according to the respective positions of the nucleoprotein (N) and glycoprotein (G) genes along the genome, ...
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A nonrad~oac t~ve DNA probe assay was developed to detect and ~ d e n t ~ f y infect~ous hernatopoiet~c necrosls virus (IHNV) uslng a dot blot format The probe a synthet~c DNA oligonuc leot~de labeled enzymatlcally w ~ t h biotln hybnd~zed spec~f~cal ly w ~ t h nucleocaps~d mRNA extracted from Infected cells early In the vlrus repl~cation cycle A r a p ~ d g u a n ~ d l n ~ u m th~ocyanate base...
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Infectious hematopoietic necrosis (IHN)-a highly lethal infectious salmonid disease-has caused substantial economic losses in the European production of rainbow trout (Oncorhynchus mykiss) since the late 1980s. The causal agent of IHN is the IHN virus (IHNV) introduced from overseas. However, until today, its phylogeographic spread in Europe remains poorly understood. We therefore sought to elu...
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ژورنال
عنوان ژورنال: Fisheries science
سال: 2002
ISSN: 0919-9268
DOI: 10.2331/fishsci.68.sup2_1151